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| Description |
Apoptosis is a complex biological process in which the activation of one or more apoptotic
pathways leads to a series of biochemical events ultimately resulting in
cell destruction. The Apoptosis Pathway Guide siRNA Array is designed as a
simple and reliable tool to identify the apoptosis pathway(s) within the
apoptotic machinery that are critically important to your research.
Four (4) major extrinsic pathways including receptors utilizing the
death inducing signaling complex (DISC), tumor necrosis factor receptors (TNFR),
TNFR family adapters, growth factor receptors and five (5) major intrinsic
gene groups including initiator caspases, effector caspases,
anti-apoptotic BCL-2 family members, pro-apoptotic BCL-2 family members
and nuclear regulators such as p53 are represented on the Apoptosis
Pathway Guide siRNA Array. Validated siRNAs of 42 genes in respective
apoptosis pathways, and appropriate RNAi controls are arrayed on a 96-well
plate, ready to use for reverse transfection. Directly determine cell
phenotypes on the siRNA Array plate with colorimetric, luminescent or
fluorescent cell-based assays. Using the Apoptosis Pathway Guide siRNA
Array, you can quickly and reliably identify the apoptotic pathway(s) in
the complex apoptotic machinery that interact with your gene product,
pathway, or small molecule compound.
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| Functional Gene Groupings |
Key Genes in the Extrinsic Apoptosis Pathways
- Receptor Systems and Adapters Utilizing the Death Inducing Signaling Complex
(DISC)
FADD, FAS, TNFRSF10A, TNFRSF10B, CASP8
- Tumor Necrosis Factor Receptor (TNFR) Family
CD40, TNFRSF1A, TNFRSF1B, TNFRSF21, TNFRSF25
- Tumor Necrosis Factor Receptor (TNFR) PATHWAY Family Adapters
BIRC2, BIRC4, CASP8, CRADD, FADD, MAPK8, RIPK2, TRADD, TRAF2
- Survival/Growth Factor/Cytokine Receptor Systems and Adapters
AKT1, BAG1, IGF1R, BAD
Key Genes in the Caspase Cascade
- Initiator Caspases and their Regulators
APAF1, CASP8, CASP9, CASP10, CFLAR/FLIP
- Effector Caspases and their Regulators
CASP1, CASP2, CASP3, CASP6, CASP7, DIABLO
Key Genes for Bcl2 Family Members and their Regulators
- Anti-apoptotic Bcl2 Family Members
BCL2, BCL2A1, BCL2L1, MCL1
- Pro-apoptotic Bcl2 Family Members
BAD, BAK1, BAX, BCL2L1, BID, BIK
Key Genes for Nuclear Regulators of Apoptosis
BCLAF1, BIRC5, TP53
| Gene Function Summary |
| Pro-apoptotic |
BAD, FADD, BAK1, MAPK8, FAS, CASP1, CASP10, CASP2,
CASP3, CASP6, CASP7, CASP8, CASP8, CASP9, BAX, BCL2L1, BCLAF1,
CD40, DIABLO, BID, BIK, RIPK2, BIRC5, AKT1, TNFRSF10A, TNFRSF10B,
TNFRSF1A, TNFRSF1B, TNFRSF21, TNFRSF25, TP53, APAF1, TRADD, TRAF2, |
| Anti-apoptotic |
AKT1, BAG1, BCL2, BCL2A1, BCL2L1, BIRC2, BIRC4,
BIRC5, CASP8, CFLAR/FLIP, CRADD, IGF1R, MCL1 |
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| Product Resources |
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| Related Products for the Genes on This Array |
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| What is a SureSilencing siRNA Array? |
| The SureSilencing siRNA Arrays are the most convenient and reliable tools for functional studies of a complex pathway or biological network. Validated siRNAs for key genes in different locations and/or different pathways are represented in a 96-well plate. Combined with appropriate RNAi controls, they can be readily used for reverse transfection. Directly determine cell phenotypes on the same plate with colorimetric, luminescent or fluorescent cell-based assays. Studying the functions of a pathway or a biological network is made easy with SureSilencing siRNA Arrays.
Need more information about siRNA Array Products? Please SureSilencing
siRNA Array home page or send Email to Technical Support
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| Storage Conditions |
| Please check the kit components immediately after you receive this package. SuperArray is only responsible for missing items reported within two (2) business days of receipt.
Storage Conditions: SureSilencing siRNA Arrays are
shipped at ambient temperature. Keep plates at 4 ºC for
long-term storage. The arrays are stable for six (6) months at the
recommended temperature. Do not use kit beyond the expiration date
printed on the label.
This product is made under license from The Carnegie Institution of Washington. However, the purchase of this material by a non-academic or for-profit organization will require a license to use the material from Carnegie. License queries may be directed to Gloria Brienza or Gary Kowalczyk at The Carnegie Institution of Washington, 1530 P Street NW, Washington, DC 20005.
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