The SureSilencing™ Pre-Designed shRNA Plasmids specifically knock down the expression of every human, mouse or rat gene by RNA interference. For each gene, we provide four plasmids each with a different pre-designed short hairpin RNA (shRNA) sequence. Our proprietary experimentally verified shRNA design algorithm insures the maximum gene-specificity and efficacy. At least two of the four pre-designed shRNA plasmids are guaranteed* to knock down expression of the targeted gene. The availability of two effective sequences allows you to properly control for non-specific and off-target effects.

Search SureSilencing™ shRNA:

Enrich or Select: Plasmids are available with either GFP Marker or Neomycin or Puromycin Resistance, enabling enrichment and the study of the long-term effects of gene suppression.
GUARANTEED!*: Knock down expression of the targeted gene by at least 70 percent. Control for non-specific and off-target effects.
Convenient & Cost-Effective: Use standard plasmid-based and lipid-mediated transfection methods. Plasmids provide a renewable source of RNA Interference.

We offer three different plasmid formats for the SureSilencing™ shRNA. Plasmids containing the neomycin  or puromycin resistance gene let you select for stably transfected cells and study long-term effects of gene knock-down in your cells. Plasmids containing the GFP gene offer a better alternative to siRNA letting you identify and track transiently transfected cells and study short-term effects in a transient transfection. The ability to track or stably express shRNA brings RNA interference to more-difficult-to-transfect cells as well as more easily transfected cells.

Unlike chemically synthesized siRNA, plasmid-based shRNA provides a renewable source of RNA interference. You can amplify enough plasmid in your own lab to complete your project. Purchase of our plasmids also includes complete shRNA sequence information.

For a complete overview of the SureSilencing technology, click [here].
For a brief overview of the protocol and its potential applications, click [here].

SuperArray provides a full range of solutions for your RNA Interference needs:

• Search for pre-designed plasmids containing shRNA for your human, mouse, or rat gene of interest [here].

• Read [this] about the reagents & equipment required to use the RNA Interference in your lab.

• Search [here] for the RT² PCR Primer Set for the target gene to verify suppression.

• For SureSilencing Plasmid Pricing and other Ordering Instructions, click [here].

* At least two of the provided pre-designed SureSilencing™ shRNA Plasmids are guaranteed to knock down expression of the targeted gene at the RNA level by at least 70 percent as measured by real-time qRT-PCR in transfected cells upon FACS-based enrichment for GFP expression or selection for neomycin or puromycin resistance as described in the User Manual.

This product is made under license from The Carnegie Institution of Washington. However, the purchase of this material by a non-academic or for-profit organization will require a license to use the material from Carnegie. License queries may be directed to Gloria Brienza or Gary Kowalczyk at The Carnegie Institution of Washington, 1530 P Street NW, Washington, DC 20005.