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All services include confirmation of RNA sample concentration and quality.
Concentration: NanoDrop spectrophotometer
Quality: Agilent 2100 BioAnalyzer with a
RNA 6000 NanoChip
All electronic files containing results are downloaded from a secure FTP web
site, except for the Affymetrix GeneChip® Service whose results are mailed on a
CD.
GEArray® Focused DNA Microarrays
RT² Profiler™ PCR Arrays
RT² Real-Time PCR
RNA Isolation Services
Agilent Gene Expression DNA Microarrays
Affymetrix™ GeneChips®
GEArray® Focused DNA
Microarrays:
Unbiased synthesis of
biotin-labeled target using our most accurate and sensitive methods optimized
for our arrays
Hybridization of labeled targets on the pathway-focused GEArray of your choice
Find the right cataloged pathway-focused GEArray for your research
[here].
Learn how to design a custom GEArray tailored to your specific research
[here].
Sensitive chemiluminescent detection using our optimized buffers and
experimental conditions as well as a CCD camera that provides the widest
possible dynamic ranges
Clear and thorough data analysis using pair-wise comparisons
Analysis performed with our GEArray Expression Analysis Suite software
Includes microarray images, exported data, and scatter plots RT² Profiler™ PCR Arrays Unbiased
reverse transcription of RNA into cDNA template using our ReactionReady™ First
Strand cDNA Synthesis Kit (C-01)
Reliable and reproducible characterization on the pathway-focused PCR Array of
your choice
Find the right cataloged pathway-focused PCR Array for your research
[here].
Learn how to design a custom PCR Array tailored for your specific project
[here].
Clear and thorough data analysis using pair-wise comparisons in a Microsoft
Excel template
Includes raw data and tabular results as well as Scatter, 3D, and Volcano Plots RT² Real-Time PCR Unbiased
reverse transcription of RNA into cDNA template using our ReactionReady™ First
Strand cDNA Synthesis Kit (C-01)
Includes a no reverse transcription control reaction for each sample
Clear and thorough data analysis in Microsoft Excel
Choose from one of two available PCR Services:
Quantitative PCR Services Using the Calibration Curve Method:
Generation of calibration curve for each gene of interest and your choice of a
housekeeping gene
Uses our XpressRef™ Universal Reference RNA
Duplicate curves with five orders of magnitude
Choose an appropriate housekeeping gene [here]
Technical triplicates of two different template amounts for each gene in each
sample
Quantitative calibrated determination of normalized changes in expression
Quantitative PCR Services Using the ΔΔCt Method:
Technical triplicates for each gene including a housekeeping gene in each sample
Use of ΔΔCt method to calculate normalized expression changes
Accuracy insured by high amplification efficiencies
RNA
Isolation Services Standard
RNA Isolation Services:
We can accept the following biological source material: Cells (transformed,
cultured cells or primary cells), Tissue Samples or Biopsies (fresh or frozen),
Blood, and others. Contact a Technical Support representative for more
information.
Specialized RNA Isolation Services:
After a free comprehensive consultation, we can also accept FFPE samples (Formalin-Fixed
Paraffin Embedded), LCM samples (Laser Capture Micro-dissection), Fine Needle
Biopsies (FNB), and other small samples. Contact a Technical Support
representative for more information.
All RNA Isolation Services include:
Confirmation of RNA sample concentration on a NanoDrop spectrophotometer and
RNA quality on an Agilent 2100 BioAnalyzer with a RNA 6000 NanoChip
Clear and through analysis of RNA quality
Secure FTP web site for downloading all electronic
files containing your results
Direct use in one our expression analysis services:
RT² Profiler PCR Array
SABiosciences GEArray®
RT² Real-Time PCR
Agilent DNA Microarrays
Affymetrix GeneChip®
NOTE: LCM, FNB, and other small samples currently can only be characterized by
our microarray expression analysis services together with the
TrueLabeling-PicoAMP™ Service. For real-time PCR Service on these samples, contact Technical Support.
Agilent Gene Expression DNA Microarrays
Confirmation of RNA sample concentration on a NanoDrop spectrophotometer and RNA quality on an Agilent 2100 BioAnalyzer with a RNA 6000 NanoChip
Your choice of:
One-color experiments, one sample per array
Two-color experiments with universal RNA normalization, one sample per array
Two-color experiments for pair-wise comparisons, two samples per array
Synthesis of Cy3- and/or Cy5-labeled target using an amino allyl method
Purification and quality control check of the labeled target
Length determination on an Agilent 2100 BioAnalyzer with a RNA 6000 NanoChip
Quality-controlled pre-synthesized and labeled target may also be accepted for hybridization service only. Please contact a Technical Sales representative for more information.
Hybridization, after optimal target fragmentation, to the DNA Microarray of your choice
Sensitive fluorescent detection to collect raw expression profile data using the Agilent 2565A scanner
Data output as a text file containing gene-annotated raw and image normalized data
Secure FTP web site for downloading all electronic files containing your results
Affymetrix™ GeneChips®
Unbiased synthesis of biotin-labeled target using the recommended Enzo method
Synthesis and cleanup of double-stranded cDNA from the RNA samples
Preparation of biotinylated cRNA using the standard IVT-based method
Purification and quality control check of the biotin-labeled target
Length determination by gel electrophoresis
Optimal cRNA fragmentation and quality control check
Length determination by gel electrophoresis
Hybridization to the GeneChip of your choice provided separately
Sensitive fluorescent detection to collect raw expression profile data using the
Agilent 2500A scanner
Clear and thorough data analysis using Microarray Suite 5.0
All available files exported from Microarray Suite 5.0 (_annot, .cel, .chp,
.exp, .xls, files)
GeneChip® is a registered trademark of Affymetrix.
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