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TrueLabeling-AMP™ 2.0 is designed to rapidly amplify and label
antisense RNA for hybridization to the Oligo GEArray® from SABiosciences
Bioscience. Starting from total RNA, this kit utilizes a proprietary and
patent-pending linear RNA amplification and labeling procedure to
synthesize labeled antisense RNA (aRNA), also known as labeled cRNA
target. The method provides a very simple and robust procedure that
requires only one tube, without any intermediate cleanup steps, and less
than three hours to generate enough labeled cRNA for Oligo GEArray®
hybridization when starting with more than 1.0 µg of total RNA.
Alternatively, when starting with as little as 100 ng of total RNA, the
amplification time may be lengthened to increase the yield in order to
obtain enough cRNA to perform Oligo GEArray® analysis. TrueLabeling-AMP™
2.0 has been specifically designed for the Oligo GEArray® and
optimized for improved sensitivity while maintaining the representation of
the original RNA.
For a complete overview of the TrueLabeling-AMP™ method, click [here]
Benefits & Features of the TrueLabeling-Rapid AMP™ 2.0 Kit:
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Fast: 2 1/2 hour cRNA amplification and
labeling method
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Easy: One tube procedure that requires no intermediate
cleanup steps
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Sensitive: Improved sensitivity on Oligo GEArray®
hybridizations
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Reliable: Linear amplification maintains the
representation of the original RNA
For more information, see the Product
Information Sheet or the User
Manual.
Be sure to use the TrueLabeling-AMP™ 2.0 Kit in conjunction with our:
ArrayGrade™ cRNA Cleanup
The ArrayGrade™ cRNA Cleanup kit purifies labeled
cRNA from other reaction components following the TrueLabeling-AMP™
2.0 Kit protocol before hybridization. The special silica
membrane spin column technology used in the kit makes the procedure fast
and easy to perform with less than 30 minutes hands-on-time at room
temperature. The column easily binds all of the cRNA, washes away all
other material, and elutes pure cRNA in a small easily managed volume for
hybridization to the Oligo GEArray of your choice.
Silica Membrane Spin Column Technology:
High binding capacity and small elution volume
Fast and easy to perform:
Less than 30 minutes hands on time at room temperature
For more information, see the Product
Information Sheet or the TrueLabeling-AMP
User Manual.
The Oligo
GEArray Starter Kit and the Oligo
GEArray Reagent Kit include all reagents and materials for both the
TrueLabeling-AMP™ and the ArrayGrade™ Cleanup Kits. Click [here]
for more information.
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