Reagents for Oligo GEArrays®:

1. Target Labeling, Target Cleanup, Hybridization, and Detection:
   a. Complete Reagent Kits: Include everything needed for the experiment (except biotin-UTP)

   Oligo GEArray Starter Kit (Catalog Number GA-029) for 4 GEArrays OR
   Oligo GEArray Reagent Kit (Catalog Number GA-034) for 12 GEArrays OR
   GEArray Express Bulk Kit (Contact a Sales Representative for Availability) for 96 GEArrays

   b. Individual Regent Components: for 12 GEArrays each

   TrueLabeling-AMP 2.0 Kit (Catalog Number GA-030)
   ArrayGrade™ cRNA Cleanup Kit (Catalog Number GA-012)
   GEAhyb Hybridization Solution (Catalog Number H-01)
   Chemiluminescent Detection Kit (Catalog Number D-01)

2. RNA Isolation:
   The optimal RNA isolation method depends on the nature of your biological source material. Click [here] for a complete set of recommendations.
3. Target Labeling: Biotin-UTP (10 mM)
            Either: Perkin Elmer, Biotin-11-UTP: Catalog # NEL543001EA
                    Use promotion code SA2004 for a 30% discount off list price
            Or      Roche Applied Science, Biotin-16-UTP: Catalog # 11388908910
            Or      Enzo Life Sciences, Biotin-16-UTP: Catalog # 42814

Equipment for Oligo GEArrays®:

1. PCR Machine (Thermal Cycler) or multiple water baths for sample / target labeling
2. Hybridization Oven and Washing (one of the following):
   1. GEArray® Express Thermoshaker (Oligo GEArray HybPlate format, standard protocol)
   2. Hybridization Oven, rotisserie-type (Oligo GEArray HybTube format)
   3. Standard 60 ºC Oven (Oligo GEArray HybPlate format, alternative protocol)
3. Microarray image acquisition:
   1. CCD Camera:  Alpha Innotech FluorChem Series or Syngene GeneGnome or ChemiGenius Series
   2. OR X-ray film: Kodak, BioMax MR or BioMax Light for chemiluminescent detection
4. GEArray Expression Analysis Suite for data acquisition and analysis