Labeling samples for microarray hybridization is a critical factor in array analysis, second only to RNA preparation. The more abundant, specific and labeled the sample is, the more sensitive and accurate the array analysis will be. Conventional methods of sample labeling are non-specific and result in a high background and yield weak or false-positive signals. Consequently, the observed relative gene expression profile becomes skewed and unreliable.

These conventional methods also require large amounts of total RNA and detect only medium to high abundance messages. When RNA is more limiting or when studying low abundance messages, amplification methods are required. The use of some amplification methods to increase array sensitivity involves cumbersome steps and also misrepresents the relative expression profile.

Our sample labeling kits, optimized for use with our GEArray™ products, avoid these difficulties without compromising sensitivity or specificity in your microarray analysis. These kits improve the limits of detection and provide a more accurate representation of relative gene expression profiles. The linear amplification methods are quicker and easier to perform than other procedures and increase signal intensities without skewing the expression profile.

TrueLabeling-PicoAMP™ 2.0 Kit	Catalog Number GA-130
Recommended for small RNA amounts on the Oligo GEArray and high-density genome-wide microarrays
Technology:	Two-Round RNA Amplification & cRNA Target Labeling
Designed for Array Type:	Oligo (Catalog # OHS, OMM, ORN, EHS, EMM, ERN)
Labeled Nucleotide:	Biotin-UTP (Oligo GEArray & Affymetrix GeneChip Chemiluminescent Detection) Or Aminoallyl-UTP (Agilent Fluorescent Detection)
TrueLabeling-AMP™ 2.0 Kit	Catalog Number GA-030
Recommended Method for Oligo GEArray
Technology:	Synthesis, Labeling, and Amplification and cRNA Target
Designed for Array Type:	Oligo (Catalog number starting with OHS, OMM, ORN)
Labeled Nucleotide:	Biotin-UTP (Chemiluminescent Detection Only)