Benefits of the TrueLabeling-PicoAMP Kit:

• Sensitive: Use microarrays with as little as 20 cells or 50 pg of RNA
• Convenient: Easy Two-Reaction, Two-Tube, Two-Purification Protocol
• Accurate: Low amplification bias maintains original expression levels

Greater Sensitivity without Compromising the Original Profile

The TrueLabeling-PicoAMP Kit is designed to perform two rounds of amplification and labeling of antisense or complimentary RNA (cRNA) target for hybridization to high-density genome microarrays like the Agilent Gene Expression DNA Microarrays or the pathway-focused Oligo GEArray® from SABiosciences. Start from as little as 20 cells or 50 pg of total RNA to generate enough labeled cRNA for microarray hybridization. The two rounds of amplification improve sensitivity from these samples. It provides the same positive call rate as larger amounts of cells or RNA using standard one-round methods. The linear RNA amplification and labeling procedure of TrueLabeling-PicoAMP™ also maintains the original gene expression profile.

For a complete overview of this technology, read our TrueLabeling-PicoAMP White Paper.

A Kit with an Easier Protocol

The TrueLabeling-PicoAMP Kit utilizes a simpler protocol than other products. The protocol involves only two purification steps and two single-tube reactions. It also takes less time to execute, only hours of hands-on time from start to finish over two days. Just add biotinylated or aminoallyl nucleotide and an RNase H Minus reverse transcriptase and you’re ready to generate labeled cRNA for your microarray hybridization from picogram quantities of total RNA.

For more information, see the Product Information Sheet or the User Manual.