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Wells A1 through G12 contain primers for genes from the same biological pathway or the same disease state or genes that are otherwise functionally related.
Wells H1 through H5 contain a housekeeping gene panel to normalize PCR Array
data.
The product information included with each cataloged PCR Array contains a list
of the pathway-focused and housekeeping genes on the array.
Well H6 contains a Genomic DNA Control (GDC) primer set that specifically
detects non-transcribed genomic DNA contamination with a high level of
sensitivity.
Wells H7 through H9 contain replicate Reverse Transcription Controls (RTC) to
test the efficiency of the RT² First Strand Synthesis Kit (C-03) reaction
with a primer set to detect template synthesized from the kit's built-in
external RNA control.
Wells H10 through H12 contain replicate Positive PCR Controls (PPC) to test
the efficiency of the polymerase chain reaction itself using a pre-dispensed
artificial DNA sequence and the primer set that detects it.
The two sets of replicate control wells (RTC and PPC) also test for
inter-well, intra-plate consistency.
The 384-well format of the PCR Arrays includes four replicates of the same
96-well format, in which a two-by-two set of wells (wells labeled 1 - 4 in gray
above) contains the same primer sets represented by the 96-well designations.
Custom PCR Arrays have your specified layout, and the product information
enclosed with the array reiterates that layout and the genes included.
PCR Array 384HT
Wells A1 through P10 (1-370) each contain a real-time PCR assay for a gene
from the same biological pathway or disease state panel of 370 genes represented
by the complete array. Wells P11 through P15 contain a housekeeping gene panel
to normalize PCR Array data. The product information included with each
cataloged PCR Array contains a list of the pathway-focused and housekeeping
genes on the array.
Wells P16 through P18 contain replicate Genomic DNA Control (GDC) primer sets
that specifically detect non-transcribed genomic DNA contamination with a high
level of sensitivity.
Wells P19 through P21 contain replicate Reverse Transcription Controls (RTC)
to test the efficiency of the RT² First Strand Kit (C-03) reaction with a primer
set to detect template synthesized from the kit's built-in external RNA control.
Wells P22 through P24 contain replicate Positive PCR Controls (PPC) to test
the efficiency of the polymerase chain reaction itself using a pre-dispensed
artificial DNA sequence and the primer set that detects it.
The three sets of replicate control wells (GDC, RTC, and PPC) also test for
inter-well, intra-plate consistency.
Custom PCR Arrays have your specified layout, and the product information
enclosed with the array reiterates that layout and the genes included.
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