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Using total RNA in real-time PCR assays for miRNA performs well with some
sequences and some cell lines and tissues. For most other miRNA sequences and
biological material, the presence of the other RNA species besides miRNA
amplifies secondary products, increases the non-specific background, and
diminishes sensitivity and dynamic range. The RT² qPCR-Grade miRNA Isolation Kit
brings miRNA profiling to wider variety of labs and research interests by
specifically isolating small RNA enriched for miRNA. The exclusive use of small
RNA to profile miRNA eliminates the amplification of non-specific products from
irrelevant RNA. The RT² qPCR-Grade miRNA Isolation Kit helps maintain the
specificity and sensitivity expected of real-time PCR for all miRNA sequences in
many more types of samples.
Benefits of the RT² qPCR-Grade™ miRNA Isolation Kit:
- High Quality and High
Yield: Isolate enough high-quality miRNA from cells and tissues
with a protocol optimized for real-time RT-PCR expression analysis
- Reliable and Sensitive:
Find more miRNA sequenes expressed at lower levels while eliminating
false positives
- Fast and Convenient: Go from as many as 12 samples to
purified miRNA with less than 60 minutes of hands-on time
Better RNA quality means better real-time RT-PCR results.
Micro RNA quality is one of the most important factors for successful
expression analysis by real-time RT-PCR. SABiosciences has years of
experience helping researchers who are new to expression analysis
technologies and who wish to use a wide variety of sample sources. We
know what it takes to get miRNA of sufficient quantity and quality for
real-time PCR experiments. SABiosciences has put this level of expertise
into its RT² qPCR-Grade™ miRNA Isolation Kit for researchers to take
advantage of our experience and perform their experiments right the
first time.
HOW IT WORKS:
SABiosciences' RT² qPCR-Grade™ miRNA Isolation Kit is optimized for real-time
RT-PCR based gene expression analysis. It is designed to extract up to 10
µg of miRNA that is free of genomic DNA, large RNA, and other impurities.
The protocol works in combination with a phenol/chloroform-based extraction
method for cultured cells and animal tissues.
The special silica membrane spin column technology, with appropriate ethanol
concentrations, permits small RNA to flow-through while longer RNA and DNA bind
an initial column. Re-adjusting the ethanol concentration in this eluate then
permits optimal retention of the miRNA on a second equivalent column. Washing
the second column removes salts, metabolites, and other macromolecular cellular
components. Low ionic strength conditions finally elute pure miRNA ready for
subsequent cDNA template synthesis with the RT² miRNA First Strand Kit (MA-03).
The RT² qPCR-Grade™ miRNA Isolation Kit provides an easy and efficient approach
to acquire miRNA of a greater purity and higher yield. The protocol takes only
60 minutes of hands-on time, and the kit includes sufficient materials to purify
12 RNA samples. RNA purified by this kit has been thoroughly tested on and
optimized for our RT² miRNA qPCR System.
For more information, see our RT² qPCR-Grade miRNA Isolation Kit User Manual [here],
or see the RT² qPCR-Grade miRNA Isolation Kit Specification Sheet [here].
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