| Description |
The CASE™ Kit for ATF2 is designed to analyze ATF2 phosphorylation in cultured mouse cell lines. This cell-based ELISA kit quantifies the amount of activated (phosphorylated) ATF2 protein relative to total ATF2 protein. The phosphorylation sites are threonines 69 and 71. By determining ATF2 protein phosphorylation in your experimental model system, you can verify ATF pathway activation in your cell line and determine the effect of various treatments, inhibitors, or activators on the pathway.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
More information on the ATF2 protein from NCBI:
"This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined."
Reference: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?dopt=GenPept&cmd=Retrieve&db=protein&list_uids=22538422
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| Useful Links |
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| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Storage Conditions: |
Box 1 is shipped at either ambient or a
refrigerated temperature and should be stored at 4 ºC. Box 2 is shipped on dry ice or cold packs
and should be stored at -20 ºC. |
Shelf Life: All reagents are stable at the recommended
temperature for 6 months after receipt of the kit.
Enough reagents are provided for 96 assays.
| Box 1: Assay and Detection
Reagents |
Box 2:
Antibodies¹ |
| (Store at 4 ºC) |
(Store at -20 ºC) |
| Components |
Components |
Dilution Factor |
| 10X Antigen Retrieval Buffer² |
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| 10X Washing Buffer² |
Anti-Phospho-Protein Specific Antibody |
1:100 |
| Antibody Dilution Buffer |
Anti-Pan-Protein Specific
Antibody |
1:100 |
| Blocking Buffer, dry powder³ |
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| Cell Staining Solution |
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| Developing Solution |
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Secondary Antibody¹
(Dilute
1:16 before use.)
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| Stop Solution |
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| Washing Buffer Reservoir (4) |
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¹ Each antibody stock must be freshly diluted by the
appropriate factor into Antibody Dilution Buffer on the same day of
the experiment. See the CASE™ Kit User Manual for more details.
² 10X Washing Buffer and 10X Antigen Retrieval Buffer must be
diluted to 1X with dH2O before use.
³ Just before use, dissolve powder in 30 ml 1X Washing Buffer by
mixing well. Once dissolved, Blocking Buffer may be stored at 4 ºC
for up to two (2) weeks.
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