| Description |
The CASE™ Kit for SRC is designed to analyze SRC phosphorylation in cultured human and mouse cell lines. This cell-based ELISA kit quantifies the amount of activated (phosphorylated) SRC protein relative to total SRC protein. The SRC phosphorylation site is tyrosine 418. By determining SRC protein phosphorylation in your experimental model system, you can verify SRC pathway activation in your cell line and determine the effect of various treatments, inhibitors, or activators on the pathway.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
More information on SRC protein from NCBI:
The gene encoding SRC, a non-receptor tyrosine kinase, "is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein ... is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase." SRC can be activated by several growth factors, such as PDGF, M-CSF and G-CSF. The kinase activity of SRC is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr418 in the catalytic SH2 domain up-regulates enzyme activity. Phosphorylation of Tyr527 in the carboxyl-terminal tail by CSK negatively regulates its activity.
Reference: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=38202215 |
| Useful Links |
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| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Storage Conditions: |
Box 1 is shipped at either ambient or a
refrigerated temperature and should be stored at 4 ºC. Box 2 is shipped on dry ice or cold packs
and should be stored at -20 ºC. |
Shelf Life: All reagents are stable at the recommended
temperature for 6 months after receipt of the kit.
Enough reagents are provided for 96 assays.
| Box 1: Assay and Detection
Reagents |
Box 2:
Antibodies¹ |
| (Store at 4 ºC) |
(Store at -20 ºC) |
| Components |
Components |
Dilution Factor |
| 10X Antigen Retrieval Buffer² |
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| 10X Washing Buffer² |
Anti-Phospho-Protein Specific Antibody |
1:100 |
| Antibody Dilution Buffer |
Anti-Pan-Protein Specific
Antibody |
1:100 |
| Blocking Buffer, dry powder³ |
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| Cell Staining Solution |
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| Developing Solution |
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Secondary Antibody¹
(Dilute
1:16 before use.)
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| Stop Solution |
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| Washing Buffer Reservoir (4) |
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¹ Each antibody stock must be freshly diluted by the
appropriate factor into Antibody Dilution Buffer on the same day of
the experiment. See the CASE™ Kit User Manual for more details.
² 10X Washing Buffer and 10X Antigen Retrieval Buffer must be
diluted to 1X with dH2O before use.
³ Just before use, dissolve powder in 30 ml 1X Washing Buffer by
mixing well. Once dissolved, Blocking Buffer may be stored at 4 ºC
for up to two (2) weeks.
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