| Description |
The CASE™ Kit for PIK3R1 is designed to analyze PI-3-Kinase activation via tyrosine receptor kinases in cultured human and mouse cell lines. This cell-based ELISA kit quantifies the amount of activated (phosphorylated) tyrosine receptor kinases relative to total PI-3-Kinase p85 regulatory subunit protein. The phosphorylation site is the tyrosine in the p85-binding consensus motif (YxxM) of any tyrosine receptor kinase. By determining tyrosine receptor kinase activation in your experimental model system, you can verify PI-3-Kinase pathway activation in your cell line and determine the effect of various treatments, inhibitors, or activators on the pathway.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
More information on PIK3R1 protein from NCBI:
"Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of 85kD. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance."
Reference: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=32455251 |
| Useful Links |
|
|
| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Storage Conditions: |
Box 1 is shipped at either ambient or a
refrigerated temperature and should be stored at 4 ºC. Box 2 is shipped on dry ice or cold packs
and should be stored at -20 ºC. |
Shelf Life: All reagents are stable at the recommended
temperature for 6 months after receipt of the kit.
Enough reagents are provided for 96 assays.
| Box 1: Assay and Detection
Reagents |
Box 2:
Antibodies¹ |
| (Store at 4 ºC) |
(Store at -20 ºC) |
| Components |
Components |
Dilution Factor |
| 10X Antigen Retrieval Buffer² |
|
|
| 10X Washing Buffer² |
Anti-Phospho-Protein Specific Antibody |
1:100 |
| Antibody Dilution Buffer |
Anti-Pan-Protein Specific
Antibody |
1:100 |
| Blocking Buffer, dry powder³ |
|
|
| Cell Staining Solution |
|
|
| Developing Solution |
|
|
Secondary Antibody¹
(Dilute
1:16 before use.)
|
|
|
| Stop Solution |
|
|
| Washing Buffer Reservoir (4) |
|
|
¹ Each antibody stock must be freshly diluted by the
appropriate factor into Antibody Dilution Buffer on the same day of
the experiment. See the CASE™ Kit User Manual for more details.
² 10X Washing Buffer and 10X Antigen Retrieval Buffer must be
diluted to 1X with dH2O before use.
³ Just before use, dissolve powder in 30 ml 1X Washing Buffer by
mixing well. Once dissolved, Blocking Buffer may be stored at 4 ºC
for up to two (2) weeks.
|
