| Description |
The CASE™ Kit for IκBα is designed to analyze IκBα phosphorylation in cultured human cell lines. This cell-based ELISA kit quantifies the amount of activated (phosphorylated) IκBα protein relative to IκBα total protein. The IκBα phosphorylation site is serine 32. By determining IκBα protein phosphorylation in your experimental model system, you can verify NFκB pathway activation in your cell line and determine the effect of various treatments, inhibitors, or activators on the pathway.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
More information on IκBα protein from NCBI:
"NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA..."
Reference: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=48928016 |
| Useful Links |
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| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Storage Conditions: |
Box 1 is shipped at either ambient or a
refrigerated temperature and should be stored at 4 ºC. Box 2 is shipped on dry ice or cold packs
and should be stored at -20 ºC. |
Shelf Life: All reagents are stable at the recommended
temperature for 6 months after receipt of the kit.
Enough reagents are provided for 96 assays.
| Box 1: Assay and Detection
Reagents |
Box 2:
Antibodies¹ |
| (Store at 4 ºC) |
(Store at -20 ºC) |
| Components |
Components |
Dilution Factor |
| 10X Antigen Retrieval Buffer² |
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| 10X Washing Buffer² |
Anti-Phospho-Protein Specific Antibody |
1:100 |
| Antibody Dilution Buffer |
Anti-Pan-Protein Specific
Antibody |
1:100 |
| Blocking Buffer, dry powder³ |
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| Cell Staining Solution |
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| Developing Solution |
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Secondary Antibody¹
(Dilute
1:16 before use.)
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| Stop Solution |
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| Washing Buffer Reservoir (4) |
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¹ Each antibody stock must be freshly diluted by the
appropriate factor into Antibody Dilution Buffer on the same day of
the experiment. See the CASE™ Kit User Manual for more details.
² 10X Washing Buffer and 10X Antigen Retrieval Buffer must be
diluted to 1X with dH2O before use.
³ Just before use, dissolve powder in 30 ml 1X Washing Buffer by
mixing well. Once dissolved, Blocking Buffer may be stored at 4 ºC
for up to two (2) weeks.
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