| Description |
| Bay 11-7085,
(2E)-3-[[4-(1,1-Dimethylethyl)phenyl]sulfonyl]-2-propenenitrile, is an
irreversible inhibitor of TNFa activated IkBa phosphorylation. The
reported IC50 is 10 mM. It is supplied as 50 mM solution in DMSO, a
convenient format for using SABiosciences' cell-based ELISA assays, the
CASE™ kits.
Quality Control Assay: Cells pre-treated without or with inhibitor at
20 µM for 1 hour and then also treated with 20 ng/ml TNF were
assayed for pathway activation with the CASE™ Kit for IkBa S32/S36.
Based on the concentration used for quality control purposes, this
product contains enough material for at least 200 assays. However, the
optimal conditions for each specific application should be determined
empirically, and the number of assays achievable will vary.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
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| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Kit Contents: |
One vial |
| Storage Conditions |
This kit is shipped on dry ice. Aliquot and store
at -20 °C. Avoid repeated freezing and thawing. |
Shelf Life: Reagent is stable at the recommended
storage temperature for 1 month after receipt. |
| References: |
- Pierce et al. (1997) Novel inhibitors of cytokine-induced
IkBa phosphorylation and endothelial cell adhesion molecule expression
show anti-inflammatory effects in vivo. J Biol Chem 272, 21096.
- Hu et al. (2001) An IkBa inhibitor causes leukemia cell death
through a p38 MAP kinase-dependent, NF-kB-independent mechanism.
Cancer Res 61, 6290.
- Scaife et al. (2002) Nuclear factor kB inhibitors induce
adhesion-dependent colon cancer apoptosis: implications for
metastasis. Cancer Res 62, 6870.
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| NOTE to Customers Outside the USA: |
| This kit can only be shipped to customers within the USA and to our
International Distributors. Please contact your local distributor for pricing
and availability information. We cannot ship this Kit to customers outside the
USA without a local distributor. |
