| Description |
| SP600125, anthra[1-9cd]pyrazol-6(2H)-one,
is a selective inhibitor of JNK. The reported IC50 is 40 nM for
JNK1 and JNK2, 90 nM for JNK3. It is supplied as 20 mM solution in DMSO, a
convenient format for using SABiosciences' cell-based ELISA assays, the
CASE™ kits.
Quality Control Assay: Cells pre-treated without or with
inhibitor at 100 µM for 2 hours and then also treated with 0.5 M
sorbitol for 30 minutes were assayed for pathway activation with the
CASE™ Kit for JNK T183/Y185.
Based on the concentration used for quality control purposes, this
product contains enough material for at least 200 assays. However, the
optimal conditions for each specific application should be determined
empirically, and the number of assays achievable will vary.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
|
| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Kit Contents: |
One vial |
| Storage Conditions |
This kit is shipped on dry ice. Aliquot and store
at -20 °C. Avoid repeated freezing and thawing. |
Shelf Life: Reagent is stable at the recommended
storage temperature for 1 month after receipt. |
| References: |
- Han et al. (2001) c-Jun N-terminal kinase is required for
metalloproteinase expression and joint destruction in inflammatory
arthritis. J Clin Investig 108, 73.
- Bennett et al. (2001) SP600125, an anthrapyrazolone inhibitor
of Jun N-terminal kinase. Proc Natl Acad Sci USA 98, 13681.
- Schnabl et al. (2001) TAK1/JNK and p38 have opposite effects
on rat hepatic stellate cells. Hepatology 34, 953.
- Bain et al. (2003) The specificities of protein kinase
inhibitors: an update. Biochem J 371, 199.
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| NOTE to Customers Outside the USA: |
| This kit can only be shipped to customers within the USA and to our
International Distributors. Please contact your local distributor for pricing
and availability information. We cannot ship this Kit to customers outside the
USA without a local distributor. |
