| Description |
| SB202190,
4-[4-(4-Fluorophenyl)-5-(4-pyridinyl)-1H-imidazol-2-yl]phenol, is a highly
selective inhibitor of p38 MAPK. The reported IC50 is 50 nM for
p38a and 100 nM for p38ß. It is supplied as 20 mM solution in DMSO,
a convenient format for using SABiosciences' cell-based ELISA assays, the
CASE™ kits.
Quality Control Assay: Cells pre-treated without or with
inhibitor at 100 µM for 2 hours and then also treated with 10
µg/ml anisomycin for 30 minutes were assayed for pathway activation
with the CASE™ Kit for p38 T180/Y182.
Based on the concentration used for quality control purposes, this
product contains enough material for at least 200 assays. However, the
optimal conditions for each specific application should be determined
empirically, and the number of assays achievable will vary.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
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| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Kit Contents: |
One vial |
| Storage Conditions |
This kit is shipped on dry ice. Aliquot and store
at -20 °C. Avoid repeated freezing and thawing. |
Shelf Life: Reagent is stable at the recommended
storage temperature for 1 month after receipt. |
| References: |
- Jiang et al. (1996) Characterization of the structure and
function of a new mitogen-activated protein kinase (p38b). J Biol Chem
271, 17920.
- Frantz et al. (1998) The activation of p38 mitogen-activated
protein kinase determines the efficiency of ATP competition for
pyridinylimidazole inhibitor binding. Biochemistry 37, 13846.
- Nemoto et al. (1998) Induction of apoptosis by SB 202190
through inhibition of p38b mitogen-activated protein kinase. J Biol
Chem 273 16415.
- Davies et al. (2000) Specificity and mechanism of action of
some commonly used protein kinase inhibitors. Biochem J 351, 95.
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| NOTE to Customers Outside the USA: |
| This kit can only be shipped to customers within the USA and to our
International Distributors. Please contact your local distributor for pricing
and availability information. We cannot ship this Kit to customers outside the
USA without a local distributor. |
