| Description |
| U0126,
1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene, is a highly
selective inhibitor of both MEK1 and MEK2. The reported IC50 is 72 nM for
MEK1 and 58 nM for MEK2. It is supplied as 5 mM solution in DMSO, a
convenient format for using SABiosciences' cell-based ELISA assays, the
CASE™ kits.
Quality Control Assay: Cells pre-treated without or with inhibitor at
20 µM for 2 hours and then also treated with 2 µM PMA for 10
minutes were assayed for pathway activation with the CASE™ Kit for
ERK1/2 T202/Y204.
Based on the concentration used for quality control purposes, this
product contains enough material for at least 200 assays. However, the
optimal conditions for each specific application should be determined
empirically, and the number of assays achievable will vary.
Need more information about CASE kit? Please visit Protein Phosphorylation Home or send Email to Technical Support.
|
| Kit Contents / Packing List /
Storage Conditions |
Please check the kit components immediately after you receive
this package. SABiosciences is not responsible for any missing items not
reported within two (2) business days upon receipt.
| Kit Contents: |
One vial |
| Storage Conditions |
This kit is shipped on dry ice. Aliquot and store
at -20 °C. Avoid repeated freezing and thawing. |
Shelf Life: Reagent is stable at the recommended
storage temperature for 1 month after receipt. |
| References: |
- Favata et al. (1998) Identification of a novel inhibitor of
mitogen-activated protein kinase kinase. J Biol Chem 273, 18623.
- Duncia et al. (1998) MEK inhibitors: the chemistry and
biological activity of U0126, its analogs, and cyclization products.
Bioorg Med Chem Lett 8, 2839.
- Davies et al. (2000) Specificity and mechanism of action of
some commonly used protein kinase inhibitors. Biochem J 351, 95.
- Namura et al. (2001) Intravenous administraton of MEK
inhibitor U0126 affords brain protection against forebrain ischemia
and focal cerebral ischemia. Proc Natl Acad Sci USA 98, 11569.
|
| NOTE to Customers Outside the USA: |
| This kit can only be shipped to customers within the USA and to our
International Distributors. Please contact your local distributor for pricing
and availability information. We cannot ship this Kit to customers outside the
USA without a local distributor. |
