Oligo GEArray® Mouse Cell Cycle Microarray: OMM-020
 
Description
The Oligo GEArray® Mouse Cell Cycle Microarray profiles the expression of 112 genes key to cell cycle regulation. Cell cycle progression is precisely controlled by cyclin-dependent kinases (CDKs) and proteins that regulate CDKs. These CDKs and CDK-modifying proteins, including cyclins, CDK inhibitors, CDK phosphatases, and CDK kinases, are featured on the Oligo GEArray® Mouse Cell Cycle Microarray. Genes essential for DNA damage and mitotic spindle checkpoints, as well as genes in the SCF and APC ubiquitin-conjugation complexes, are also represented. This focused microarray allows you to compare expression in your experimental samples from a manageable number genes key to the cell cycle.

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Functional Gene Grouping

G1 phase and G1/S transition: Camk2a, Camk2b, Camk2d, Camk2g, Gpr132, Itgb1, Mtbp, Myb, Nfatc1, Ppp2r3a, Ppp3ca, Rhou, Skp2, Taf10, Slfn1.

S phase and DNA replication: Mcm2, Mcm3, Mcm4, Mcm5, Mcm6, Mcm7, Mki67, Mre11a, Msh2, Pcna, Rad17, Rad50, Rad51, Sumo1, Zrf2.

G2 phase and G2/M transition: Chek1, Ppm1d, Zrf2.

M phase: Brca2, Ccna1, Ccnb1, Cdc25a, Cdc25b, Cdk2, Nek2, Nek3, Npm2, Pes1, Prm1, Prm2, Rad21, Ran, Shc1, Smc1l1, Smc2l1, Stag1, Stag2, Terf1, Tnfsf5ip1, Wee1.

Cell cycle checkpoint and cell cycle arrest: Ak1, Apbb1, Apbb2, Brca2, Casp3, Cdk5rap1, Cdk5rap3, Cdkn1a, Cdkn1b, Cdkn1c, Cdkn2a, Chek1, Cks1, Ddit3, Dst, Gadd45a, Gas1, Gas2, Hus1, Inha, Macf1, Mad2l1, Mdm2, Msh2, Notch2, Pkd1, Pkd2, Pmp22, Ppm1d, Rad9, Sesn2, Sesn3, Sfn, Slfn1, Smc1l1, Tsg101.

Regulation of cell cycle: Abl1, Brca2, Ccna1, Ccna2, Ccnb1, Ccnb2, Ccnc, Ccnd1, Ccnd2, Ccnd3, Ccne1, Ccne2, Ccnf, Cdc37, Cdc45l, Cdk4, Cdkn1a, Cks1, E2f1, E2f2, E2f3, E2f4, E2f5, E2f6, Gadd45a, Itgb1, Rad9, Ran, Sfn, Shc1, Skp2, Tfdp1, Tnfsf5ip1.

Negative regulation of cell cycle: Apbb1, Apbb2, Atm, Brca1, Casp3, Cdkn2a, Cdkn2b, Cdkn2d, Gas1, Inha, Rbl1, Rbl2, Trp53, Trp63.

Storage Conditions
Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.

GEArray microarrays are shipped at ambient temperature enclosed in either a HybTube or ExpressPak Storage Box. They should be stored at -20 ºC upon receipt.

References
  1. Nurse, P. (2000) A Long Twentiety Century Of The Cell Cycle And Beyond. Cell 100: 71-80.
  2. Sherr, C. J. and Roberts, J. M. (1999) CDK Inhibitors: Positive And Negative Regulators Of G1-Phase Progression. Genes & Development 13: 1501-12.
  3. Harbour, J. W. and Dean, D. C. (2000) Rb Function In Cell-Cycle Regulation And Apoptosis. Nature Cell Biology 2: E65-7.
  4. Deshaies, R. J. (1995) The Self-Destructive Personality Of A Cell Cycle In Transition. Current Biology 7: 781-789.
  5. Gardner R. D. and Burke D. J. (2000) The Spindle Checkpoint: Two Transition, Two Pathways. Trends in Cell Biology 10: 154-8.
  6. Dasika, G. K., Lin, S. C., Zhao, S., Sung, P., Tomkinson, A., and Lee, E. Y. (1999) DNA Damage-Induced Cell Cycle Checkpoints And DNA Strand Break Repair In Development And Tumorigenesis. Oncogene 18: 7883-99.
  7. Amati, B. and Vlach, J. (1999) Kip1 Meets SKP2: New Links in Cell-Cycle Control. Nature Cell Biology 1: E91-3.
  8. Muller, H. and Helin, K. (2000) The E2F Transcription Factors: Key Regulators of Cell Proliferation. Biochim. Biophys. Acta 1470: M1-12.
  9. Bottazzi, M. E. and Assoian, R. K. (1997) The Extracellular Matrix And Mitogenic Growth Factors Control G1 Phase Cyclins And Cyclin-Dependent Kinase Inhibitors. Trends in Cell Biology 7: 348-352.
  10. Sherr C. J. and Weber J. D. (2000) The ARF/p53 Pathway. Curr Opin Genet. Dev 10: 94-99.
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