Oligo GEArray® Human Atherosclerosis

HybTube Format Cat. No.: OHS-038
HybPlate Format Cat. No.: EHS-038
 
Description
The Oligo GEArray® Human Atherosclerosis Microarray profiles the expression of 113 key genes involved in atherosclerosis. This array contains genes involved in the many biological processes involved in atherosclerosis including blood coagulation, circulation, cell-adhesion, stress response, lipid transport and metabolism, cell growth, proliferation and apoptosis. Through a simple side-by-side hybridization experiment you can determine differential gene expression between your samples.

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Functional Gene Grouping

Response to Stress:
Inflammatory Response: CCL11, CCL2, CCL20, CCL5, CCR1, CCR2, IL1R1, IL1R2, IL8, ITGB2, NFKB1, NOS3, PTGS2, SELE, SPP1, TNF, CD40 (TNFRSF5).

Response to Oxidative Stress: APOE, CCL5, SOD1, SOD2.

Response to Pest, Pathogen or Parasite: CCL2, CCL20, CCR2, CSF2, CSF3, FN1, IL13, IL1B, IL4, IL6, IL7, ITGB2, TNF, CD40 (TNFRSF5).

Response to Virus: CCL11, CCL5, IFNAR2, TNF.

Response to Wounding: CCR1, CCR2, CTGF, FN1, PDGFB, TNF, VWF.

Other Genes Related to the Response to Stress: APOA2, IFNG, PPARG, VEGF.

Apoptosis:
Anti-apoptosis: BCL2, BCL2A1, BCL2L1, BIRC3, CCL2, CFLAR, IL1A, IL2, NFKB1, SERPINB2, SPP1, TGFB1, TNF, TNFAIP3, FAS (TNFRSF6).

Induction of Apoptosis: APOE, BAX, BID, CFLAR, FAS (TNFRSF6).

Other Genes Related to Apoptosis: IL1B, IL5, ITGB2, CD40 (TNFRSF5).

Blood Coagulation and Circulation:
Blood Coagulation: CD36, F7, FGA, FGB, ITGA2, ITGB3, LPA, SERPINE1.

Circulation: APOA1, APOA4, APOB, APOE, COL3A1, DSCR1, ELN, ENG, LPA, LPL, NPY, OLR1.

Platelet Activation: PDGFA, PDGFB, PDGFRB, CD40 (TNFRSF5), VWF.

Regulation of Blood Pressure: ACE, FGA, FGB.

Adhesion Molecules:
Cell-cell Adhesion: CD44, CDH5, ICAM1, ICAM2, ITGB2, SELE, SELL, TNF, VCAM1, VEGF.

Cell-matrix Adhesion: CD44, ITGA2, ITGA5, ITGAX, ITGB2, ITGB3, ITGB5, ITGB7, SPP1.

Other Genes Involved in Adhesion: CCL11, CCL2, CCL5, CCR1, CD36, CTGF, ELN, ENG, FN1, IL8, LAMA1, SCARB1, SELPLG, THBS4, TNC, VWF.

Extracellular Matrix (ECM) Molecules:
ECM Protease Inhibitors: LPA, SERPINB2, SERPINE1.

ECM Proteases: ACE, MMP1, MMP3, MMP9.

Extracellular Matrix Structural Constituents: COL3A1, ELN, FN1.

Other Extracellular Molecules: ADFP, APOA1, APOA2, APOA4, APOB, APOE, CCL11, CCL2, CCL20, CCL5, CSF2, CSF3, CTGF, HBEGF (DTR), F7, FGA, FGB, FGF2, IFNAR2, IFNG, IL13, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, IL8, LAMA1, LCAT, LIF, LPL, NPY, PDGFA, PDGFB, PLTP, SPP1, THBS4, TNC, VEGF, VWF.

Lipid Transport and Metabolism:
Cholesterol Metabolism: ABCA1, APOA1, APOB, APOE, CETP, IL4, LCAT, LDLR.

Fatty Acid Metabolism: CD36, FABP3, LPL, PPARA, PTGS1, PTGS2.

Lipid Transport: ABCA1, APOA1, APOA2, APOA4, APOB, APOE, CETP, FABP3, LDLR, LPA, LPL, MSR1, PLTP.

Lipoprotein Metabolism: APOA1, APOA2, APOA4, APOE, LDLR, LPL.

Steroid Metabolism: NR1H3, PPARA, PPARD, PPARG, RXRA.

Other Genes Related to Lipid Metabolism: ADFP, APOA2, APOA4, APOE, LPA, PLTP.

Cell Growth and Proliferation:
Growth Factors and Receptors: CSF2, KDR, PDGFRB, SPP1.

Negative Regulation of Cell Proliferation: BCL2, FABP3, IL1A, IL1B, IL6, IL8.

Positive Regulation of Cell Proliferation: CSF1, CSF3, HBEGF (DTR), FGA, FGB, FGF2, IL2, IL3, IL5, IL6, IL7, LIF, VEGF.

Regulation of Cell Cycle: DUSP6, FGF2, IL1A, IL1B, PDGFA, PDGFB, TGFB1, TGFB2, TGFB3, VEGF.

Other Genes Involved in Cell Growth and Proliferation: CTGF, ELN, IFNG, IL13, IL4, NPY, CD40 (TNFRSF5).

Transcription Regulators:
Nuclear Receptors: NR1H3, PPARA, PPARD, PPARG, RXRA.

Other Transcription Regulators: DSCR1, EGR1, IL8, KLF2, NFKB1, SOD2, TNF, TNFAIP3.

Storage Conditions
Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.

GEArray microarrays are shipped at ambient temperature enclosed in either a HybTube or ExpressPak Storage Box. They should be stored at -20 ºC upon receipt.

References
  1. Laukkanen J, Yla-Herttuala S. Genes involved in atherosclerosis. Exp Nephrol. 2002; 10 (2): 150-63.
  2. Henriksen PA, Kotelevtsev Y. Application of gene expression profiling to cardiovascular disease. Cardiovasc Res. 2002 Apr; 54 (1): 16-24.
  3. Monajemi H, Arkenbout EK, Pannekoek H. Gene expression in atherogenesis.Thromb Haemost. 2001 Jul; 86 (1): 404-12.
  4. Depre C, Tomlinson JE, Kudej RK, Gaussin V, Thompson E, Kim SJ, Vatner DE, Topper JN, Vatner SF. Gene program for cardiac cell survival induced by transient ischemia in conscious pigs. Proc Natl Acad Sci U S A. 2001 Jul 31; 98 (16): 9336-41.
  5. Friddle CJ, Koga T, Rubin EM, Bristow J. Expression profiling reveals distinct sets of genes altered during induction and regression of cardiac hypertrophy. Proc Natl Acad Sci U S A. 2000 Jun 6; 97 (12): 6745-50.
  6. Shiffman D, Porter JG. Gene expression profiling of cardiovascular disease models. Curr Opin Biotechnol. 2000 Dec; 11 (6): 598-601.
  7. Shiffman D, Mikita T, Tai JT, Wade DP, Porter JG, Seilhamer JJ, Somogyi R, Liang S, Lawn RM. Large scale gene expression analysis of cholesterol-loaded macrophages. J Biol Chem. 2000 Dec 1; 275 (48): 37324-32.
  8. Topper JN, Cai J, Falb D, Gimbrone MA Jr. Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: cyclooxygenase-2, manganese superoxide dismutase, and endothelial cell nitric oxide synthase are selectively up-regulated by steady laminar shear stress. Proc Natl Acad Sci U S A. 1996 Sep 17; 93 (19): 10417-22.
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