| Description |
| TrueLabeling-AMP™ 2.0 is designed to
rapidly amplify and label antisense RNA for hybridization to the Oligo
GEArray® from SABiosciences. Starting from total RNA, this kit
utilizes a proprietary and patent-pending linear RNA amplification and
labeling procedure to synthesize labeled antisense RNA (aRNA), also known
as labeled cRNA target. The method provides a very simple and robust procedure
that requires only one tube, without any intermediate cleanup steps, and less
than three hours to generate enough labeled cRNA for Oligo GEArray® hybridization
when starting with more than 1.0 μg of total RNA. Alternatively, when starting with
as little as 100 ng of total RNA, the amplification time may be lengthened to increase
the yield in order to obtain enough cRNA to perform Oligo GEArray® analysis.
TrueLabeling-AMP™ 2.0 has been specifically designed for the Oligo GEArray® and
optimized for improved sensitivity while maintaining the representation of
the original RNA.
Need more information about GEArray products? please visit Microarray Home or send Email to Technical Support |
| Materials Included /
Packing List |
Please check the kit
components immediately after you receive this package. SABiosciences is
not responsible for any missing items not reported within two (2)
business days upon receipt.
Storage
Conditions:
The following kit components are shipped on
dry ice. For long-term storage, keep entire box at -20 ºC.
| TUBE |
CONTENTS |
| For cDNA Synthesis: |
| G1 |
TrueLabeling Primer |
| R1 |
RNase Inhibitor |
| G2 |
cDNA Synthesis Enzyme Mix |
| G3 |
5X cDNA Synthesis Buffer |
| H2O |
RNase-free H2O |
| For cRNA Synthesis, Labeling, and Amplification: |
| G24 |
2.5X RNA Polymerase Buffer |
| G25 |
RNA Polymerase Enzyme |
Biotin-UTP must also be purchased from another manufacturer to use this kit.
See User Manual for more details and additional reagents required.
Shelf Life: All
reagents are stable for 6 months after receipt of the kit if stored
at the recommended temperature. |
| Related Products
from SABiosciences Corporation |
Oligo GEArray® Microarrays (See
http://www.sabiosciences.com/ArrayList.php for complete listings)
ArrayGrade™ cRNA Cleanup Kit (GA-012)
ArrayGrade™ Total RNA Isolation Kit (GA-013)
XpressRef™ Universal Reference Total RNA for Human, Mouse, and Rat (GA-004, GA-005, GA-006, respectively)
RNASafer™ RNA Stabilization Reagent (GA-008)
Oligo GEArray® Starter Kit (GA-029)
Oligo GEArray® Reagent Kit (GA-034)
|
