| Description |
| The ArrayGrade RNA Isolation Kit is designed
to extract total RNA from small quantities of biological source material. This kit
is designed to prepare of up to 100 μg of total RNA from either cultured animal cells
or, in combination with a phenol/chloroform extraction method, from animal tissues.
The special silica membrane Spin Column technology used in the kit makes the procedure
fast and easy to perform with less than 15 minutes hands-on-time at room temperature.
The lysis buffer, with its chaotropic components, stabilizes and prevents degradation
of the RNA and allows optimal retention of the RNA on the spin column. The Washing Buffer removes salts, metabolites, and macromolecular cellular components. Low ionic strength conditions elute pure RNA from the column for labeled target synthesis and microarray-based gene expression analysis.
Need more information about GEArray products? please visit Microarray Home or send Email to Technical Support |
| Materials Included /
Packing List |
Please check the kit
components immediately after you receive this package. SABiosciences is
not responsible for any missing items not reported within two (2)
business days upon receipt.
Storage
Conditions:
The kit is shipped at ambient temperature.
Store the entire kit at room temperature.
| TUBE |
CONTENTS |
| G6 |
Lysis & Binding Buffer |
| G15 |
Desalting Buffer |
| G16 |
Pre-Wash Buffer |
| G17 |
Washing Buffer (Add 10 ml ethanol before
use.) |
| H2O |
RNase-free H2O |
| |
Spin Columns (12) |
| |
Collection Tubes (12) |
| |
Filter Columns (12) |
Shelf Life: All
reagents are stable for 6 months after receipt of the kit if stored
at the recommended temperature.
|
| Brief Protocol: For
Experienced Users |
First time users, please refer to the complete protocol in the TrueLabeling-AMP 2.0 User Manual.
- Add 350 μl of Lysis & Binding Buffer (G6) to cell pellet or to ground tissue in a 1.5-ml RNase-free tube.
Add to filter column and centrifuge for 1 min at 11,000 x g.
- Discard the filter column. Add 350 μl of ethanol (70%) to filtrate and mix.
- Load entire volume (700 μl) onto spin column. Centrifuge for 30 s at 8,000 x g.
- Add 350 μl of Desalting Buffer (G15) onto spin column; centrifuge again for 60 s at 11,000 x g.
- Add 200 μl of Pre-Wash Buffer (G16) onto spin column; centrifuge again for 30 s at 8,000 x g.
- Add 350 μl of Washing Buffer (G17+ethanol) onto spin column; centrifuge again for 30 s at 8,000 x g.
- Add 200 μl of Washing Buffer (G17+ethanol) onto spin column; centrifuge again for 3 min at 11,000 x g.
- Add 50 μl of RNase-free H2O to the spin column.
Incubate at room temperature for 1 min. Centrifuge for ~ 1 min at 11,000 x g.
Make sure that all of the wash passes through the filter. Repeat spin if necessary.
- Store RNA at -20 ºC for two to four days or at -80 ºC for up to six months.
|
| Related Products
from SABiosciences Corporation |
TrueLabeling-AMP™ 2.0 Kit (GA-030)
Oligo
GEArray® Microarrays
Oligo GEArray® Starter Kit (GA-029)
Oligo GEArray® Reagent Kit (GA-034)
|
