| Description |
| The ArrayGrade™ cRNA Cleanup Kit is
designed to purify labeled cRNA from other reaction components following
the TrueLabeling-AMP™ protocol. The special silica membrane
Spin Column technology used in the kit makes the procedure fast and easy to
perform with less than 30 minutes hands-on-time at room temperature. The Binding
Buffer with its chaotropic components stabilizes and prevents degradation of the
cRNA and allows optimal retention of the cRNA on the spin column. The Washing Buffer
removes salts, metabolites, and macromolecular cellular components. Low ionic strength
conditions elute pure cRNA from the column for hybridization to the microarray of
your choice.
Need more information about GEArray products? please visit Microarray Home or send Email to Technical Support |
| Materials Included /
Packing List |
Please check the kit
components immediately after you receive this package. SABiosciences is
not responsible for any missing items not reported within two (2)
business days upon receipt.
Storage
Conditions:
The kit is shipped at ambient temperature.
Store the entire kit at room temperature.
| TUBE |
CONTENTS |
| G6 |
Lysis & Binding Buffer |
| G17 |
Washing Buffer (Add 10 ml ethanol before
use.) |
| G26 |
RNase-free 10 mM Tris pH 8.0 |
| |
Spin Column (12) |
| |
Collecting Tube (12) |
| |
Elution Tube
(12) | Shelf Life: All
reagents are stable for 6 months after receipt of the kit if stored
at the recommended temperature.
|
| Brief Protocol: For
Experienced Users |
First time users, please refer to the complete protocol in the TrueLabeling-AMP 2.0 User Manual.
- Bring the cRNA sample to a final volume of 100 µl with
RNase-free H2O in a 1.5-ml RNase-free tube.
Store on ice.
- Add 350 µl Lysis & Binding Buffer (G6) to each
sample. Mix well but gently with a pipettor.
- Add 350 µl of room temperature ACS-Grade 100% ethanol. Mix
well but gently with a pipettor.
- Load each sample onto the center of separate Spin Columns.
- Insert Spin Column into Collection Tube. Centrifuge for ~ 30
sec at 8,000 x g.
- Remove column from the tube, discard the flow-through, and put
the column back into the tube.
- Apply 600 µl washing buffer (G17 + ethanol) to each
spin column. Centrifuge for ~ 30sec at 8,000 x g. Be
sure that all of the wash passes through the filter. Repeat spin
if necessary.
- Remove column from the tube, discard the flow-through, and put
the column back into the tube.
- Apply another 200 µl washing buffer (G17 + ethanol)
to each spin column. Centrifuge for ~ 3 min at 11,000 x g.
Be sure that all of the wash passes through the filter.
Repeat spin if necessary.
- Transfer each Spin Column to a fresh Elution Tube.
Be sure
that the tip of the column never touches the flow-through
material.
- To the center of each spin column, add 50 µl of room
temperature RNase-free 10 mM Tris pH 8.0 (G26).
NOTE: Be sure to visually inspect the column to insure
that the buffer is centered.
Tap the column gently if necessary
to move the water to the center.
- Incubate at room temperature for 2 min. Centrifuge for ~ 1 min
at 8000 x g.
Be sure that all of the wash passes
through the filter. Repeat spin if necessary.
Store at -20 ºC
for two to four days or at -80 ºC for up to six months or longer.
|
| Related Products
from SABiosciences Corporation |
TrueLabeling-AMP™ 2.0 (GA-030)
Oligo
GEArray® Microarrays
Oligo GEArray® Starter Kit (GA-029)
Oligo GEArray® Reagent Kit (GA-034) |
