As an illustrative example of the application of the ELISArray Kits, we monitored the time-dependent (0, 6, 18, 24, and 48 hours) patterns of Th1/Th2 cytokine induction by human peripheral blood mononuclear cells (PBMC) in response to PMA (50 µg/ml) and ionomycin (1 mg/ml). The relative amount of each of the eight cytokines was profiled at the same time using the ELISArray Kit.

Large amounts of IL-2 and IFNγ, and to a lesser extent TNFα, are induced throughout, and apparently beyond, the time course tested. Only more moderate amounts of IL4 and IL5 are induced, reaching a maximum at roughly just before 24 hours of stimulation. The even lower levels of IL10 and IL13 that are secreted also reach a maximum at 24 hours and appear to decrease again at later times. The amount of IL12 is not detectable after 48 hours of constant stimulation.