Complement your ChIP-qPCR studies with other experiments using our pathway-focused and genome-wide PCR-based gene expression profiling and/or RNA interference tools. Explore the role of other nuclear factors in the enrichment of your target factor at the queried genomic DNA region by suppressing their expression. Or verify the functional activity of the enrichment by profiling the expression of the target genes using real-time RT-PCR.

RT² Profiler PCR Arrays and RT² qPCR Primer Assays:
Verify that nuclear factor and/or RNA Polymerase II enrichment at your promoter of interest alters transcript levels of the relevant gene and/or other genes in the same biological pathway using real-time RT-PCR. The RT² qPCR Primer Assays are designed to analyze the transcript levels of individual genes, while the RT² Profiler™ PCR Arrays profile a panel of pathway-focused or disease-specific mRNAs. Both methods are optimized for high performance SYBR Green based qRT-PCR detection.

SureSilencing shRNA Plasmids:
Test whether other related nuclear or transcription factors are in the same complex as your ChIP target factor by knocking down the expression of another factor of interest and repeating your ChIP-qPCR experiment to test whether the target factor is still enriched at the promoter region of interest. The SureSilencing™ shRNA Plasmids are designed to knock down the expression of any gene in the human, mouse, or rat genome by plasmid-based RNA Interference.

Gene Expression Analysis Service Core:
Get gene expression profiling results even more quickly and easily by letting our in-house experts perform the real-time RT-PCR or PCR Array analysis for you. Stay focused on your protein-DNA interaction experiments by leaving your mRNA measurements to the experts.

Protein Phosphorylation CASE Kits and ELISA Kits:
Confirm the activation of the signal transduction pathway upstream of your nuclear or transcription factor of interest by monitoring the phosphorylation of the pathway's regulators using a cell-based in vitro assay. Or verify the induction of cytokines at the protein level caused by the observed enrichment of nuclear factors at their promoter using standard sandwich ELISA kits.

Oligo GEArray® Focused DNA Microarrays:
Verify that nuclear factor and/or RNA Polymerase II enrichment at your promoter of interest activates transcription of other genes in the same biological pathway using an alternative flexible, inexpensive and easy-to-use platform.