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Benefits of SABiosciences' SYBR® Green PCR Master Mixes:
Accuracy: Guarantees single specific products with minimal primer dimers.
Reliability: Maximizes amplification efficiencies.
Convenience: Specially formulated all-in-one mixes that match the instrument in
your lab.
Guaranteed: ChIP-qPCR Assay performance is uniquely designed for this chemistry.
To learn more about their performance, see our SYBR® Green Master Mix
Brochure.
Find the qPCR master mix for the instrument available in your lab:
RT² SYBR Green/ ROX
qPCR Master Mix:
Specifically designed for:
All ABI and Stratagene Instrumentation
Eppendorf Mastercycler® ep realplex Instruments
with ROX filter set |
| Cat. No. |
Size |
PA-012
PA-112 |
For 200
25-µl reactions (2.5 ml)
For 2000 25-µl reactions (25 ml) |
RT² SYBR Green /
Fluorescein qPCR Master Mix:
Specifically designed for:
BioRad iCylcer® MyiQ® and iQ5 Instrumentation |
| Cat. No. |
Size
|
PA-011
PA-111 |
For 200
25-µl reactions (2.5 ml)
For
2000 25-µl reactions (25 ml)
|
RT² SYBR Green qPCR
Master Mix:
Specifically designed for instrumentation that does not
require a reference dye:
BioRad (MJ Research) Opticon, Opticon 2, and Chromo 4
Roche
LightCycler® 480 System
Eppendorf Mastercycler® ep
realplex Instruments without ROX filter set |
| Cat. No. |
Size
|
PA-010
PA-110 |
For 200
25-µl reactions (2.5 ml)
For
2000 25-µl reactions (25 ml)
|
Master Mix quality plays a critical role in the performance of SYBR
Green-based real-time PCR. The key to obtaining accurate results is a
tightly-controlled hot start enzyme that eliminates the amplification of primer
dimers and other secondary products. Antibody-based technologies have worked
well in the past for most PCR applications. However, these enzymes too often
"leak" and synthesize too many non-specific amplicons to meet the more
rigorous and demanding standards set by SYBR Green real-time detection. After
years of experience in designing and experimentally testing thousands of PCR
primers for entire genomes, SABiosciences has developed a qPCR master mix
formulation that provides the most accurate SYBR Green real-time PCR results.
Unique HotStart Polymerase and Buffer Components
The RT² qPCR Master Mixes from SABiosciences offer superior specificity and
amplification efficiency thanks to our high-quality chemically-modified HotStart
Taq DNA polymerase. Hot start enzymes found in other sources of master mix, such
as the antibody-based technologies, still retain residual activity in their
"inactive" state at low temperature. Under these conditions,
non-specific priming events are extended to form templates which are then
amplified into non-specific products that artificially contribute to the SYBR
Green signal and cause false positive results. Our tightly controlled HotStart
enzyme lacks any activity before being heated and therefore minimizes the
amplification of non-specific PCR products.
The RT² qPCR Master Mix formulations also include other proprietary chemical
components that significantly minimize primer dimer formation ensuring high
amplification efficiencies for even the most difficult to amplify genomic DNA
sequences.
All-in-One Master Mixes: Instrument Reference Dyes Included
Our RT² qPCR master mixes are tailor-made and optimized for the specific real
time system in your lab. These cost-effective all-in-one PCR master mixes not
only contain SYBR Green, optimized buffer, nucleotides and our high-quality
HotStart DNA polymerase; they also include the appropriate reference dye
specifically used by individual real time systems to normalize their optics.
Just add your primer pairs and templates, and you're ready for PCR.
Complete your assays using the ChIP-qPCR Assays with the appropriate RT² qPCR
Master Mix tailor-made to match the real-time instrument in your laboratory.
SYBR® is a registered trademark of Molecular Probes.
ABI® and TaqMan® are registered trademarks of Applied
Biosystems.
iCycler® is a registered trademark of Bio-Rad Laboratories, Inc.
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