SuperArray is the leading expert in high-performance SYBR Green Quantitative RT-PCR.

Tired of designing and paying for your own PCR primers that just end up giving you low amplification efficiencies, non-specific amplicons or primer dimers? Tired of adjusting magnesium concentrations and other PCR conditions that still don't seem to help? SuperArray has years of experience in designing and experimentally testing thousands of PCR primers for entire genomes and optimizing a single universally applicable master mix formulation.

Combing these optimized qPCR master mixes and qPCR Primer Assays yields the most accurate SYBR Green real-time PCR results for every gene, every time. Let researchers who develop SYBR Green PCR techniques on daily basis provide you with the best possible analysis of your samples.

Learn more about our RT² qPCR Primer Assays [here].
Learn more about our RT² qPCR Master Mixes [here].

Features of the RT² PCR Service:

Confirmation of RNA sample concentration on a NanoDrop spectrophotometer and RNA quality on an Agilent 2100 BioAnalyzer with a RNA 6000 NanoChip

Unbiased reverse transcription of RNA into cDNA template using our First Strand Kit (C-03)
     Includes a no reverse transcription control reaction for each sample

Clear and thorough data analysis in Microsoft Excel
     Secure FTP web site for downloading all electronic files containing your results
     
Quantitative PCR Services Using the Calibration Curve Method:

Generation of calibration curve for each gene of interest and your choice of a housekeeping gene
     Uses our XpressRef™ Universal Reference RNA
     Duplicate curves with five orders of magnitude
     Choose an appropriate housekeeping gene [here]
Technical triplicates of two different template amounts for each gene in each sample
Quantitative calibrated determination of normalized changes in expression  

Quantitative PCR Services Using the ΔΔCt Method:

Technical triplicates for each gene including a housekeeping gene in each sample
Use of ΔΔCt method to calculate normalized expression changes
Accuracy insured by high amplification efficiencies

Pricing for the RT² Real-Time™ PCR Service:

Reverse Transcription prices start from $ 50 per sample and include the minus-RT control.

Calibration Curve Service:
     Calibration Curve prices start from $ 100 per gene for the calibration curve itself.
     Prices start from $ 50 for six reactions per sample per gene.

ΔΔCt Service:
     Prices for PCR reactions in technical triplicates start from $ 35 per sample per gene.

Gene quantity counts for both services must include a housekeeping control gene of your choice.

Prices include the cost of all reagents as well as service charges.
Service prices are volume-dependent. Progressive discounts are available.
Contact a Technical Support representative to discuss your specific needs.

For complete details or to get a specific quotation, please contact a Technical Support representative at support@superarray.net or 301-682-9200.

All prices are in US Dollars. Prices are subject to change at any time without notice.