For Transformation and plasmid purification:
Competent E. coli cells
     (For example, JM109 from Promega, catalog number L1001 or L2001)
LB medium, agar plates, ampicillin
Plasmid purification kit
     (For example, EndoFree Plasmid Maxi Kit form Qiagen, catalog number 12362)

For Transfection:
Transfection reagent:
     Lipofectamine2000™ (from Invitrogen, catalog numbers 11668-027/-019)
Other materials recommended by transfection reagent manufacturer:
     Opti-MEM (Gibco)
Neomycin (cell culture and/or molecular biology grade)

For Real-Time RT-PCR Verification of Gene Suppression:
ReactionReady™ First Strand cDNA Synthesis Kit (C-01)
RT² PCR Master Mix to match with your instrumentation:
     RT² Real-Time™ SYBR Green / ROX (PA-012)
          Specifically designed for ABI Real-Time Instrumentation
     RT² Real-Time™ SYBR Green / Fluorescein (PA-011)
          Specifically designed for the BioRad iCylcer®
     RT² Real-Time™ SYBR Green (PA-010)
          Specifically designed for instrumentation not requiring a reference dye
RT² PCR Primer Set targeting the suppressed gene