PCR Arrays are the most reliable tools for analyzing the expression of a
focused panel of genes. Each 96-well or 384-well plate includes SYBR Green-optimized primer
assays for a thoroughly researched panel of relevant, pathway- or
disease-focused genes. PCR Arrays can also be customized to contain a panel of
genes tailored to your specific research interests. Our high-quality primer
design and master mix formulation enable the PCR Array to amplify 96 or 384 different
gene-specific products simultaneously under uniform cycling conditions. This
combination provides the PCR Array with the specificity and the high
amplification efficiencies required for accurate real-time SYBR Green results.
The simplicity of the PCR Arrays makes them accessible for routine use in every
research laboratory.
Why PCR Arrays?
- Pathway Focused: Profile the expression
of a panel of genes relevant to a pathway or disease state.
- Simple and Accurate:
Simple real-time PCR method provides high sensitivity and wider dynamic range. Requires as little as 25 ng total RNA.
- Designed for Routine Use:
Brings expression profiling to almost any lab with a real-time PCR
instrument.
Performance
and Applications
Performance
-
Sensitivity: With
the sensitivity of the RT² First Strand Kit, as little as 25 ng or as much as
5 µg of total RNA per array plate provides greater than 80 percent
present call rates.
-
Reproducibility:
The high reproducibility of the system, with replicate correlation coefficients
> 0.99, means that experimental samples can be reliably compared across
plates and runs.
-
Specificity: The
specificity of the system, imparted by the combination of SYBR Green primers
and PCR master mixes, guarantees a single product of the predicted size from
every reaction without secondary products such as primer dimers.
The complete PCR Array System guarantees
the optimal performance of the PCR Array. Including the optional RT² RNA QC
PCR Array into the complete system maximizes control over consistent RNA
quality.
Application Examples
The PCR Arrays have been used for cancer, immunology, stem cells, toxicology, and many other areas of biological and medical research. To view application examples for cancer and toxicology research, click [ here ]. To see a list of publications citing the use of the PCR Array, click [ here
].
How It Works
Simple Workflow
Simply mix your cDNA
template with the appropriate ready-to-use PCR master mix, aliquot equal
volumes to each well of the same plate, and then run the real-time PCR cycling
program. PCR Arrays are compatible with all ABI, Bio-Rad, Stratagene and Roche
instrumentation. Find the correct setup instructions and protocol files [ here
].
Well
Designed Layout and Controls
The PCR Arrays are available in both 96- and 384-well plates and are used to
monitor the expression of 84 or 370 genes related to a disease state or pathway plus
five housekeeping genes. Controls are also included on each array for genomic
DNA contamination, RNA quality, and general PCR performance. Click [ here
] to learn how the control features work.
Easy-to-Use
Data Analysis
Download an easy-to-use Excel-based data analysis template [
here ]. Data analysis is based on the
ΔΔCt method with normalization of the raw data to either housekeeping genes or
an external RNA control.
You can easily perform
a PCR Array experiment in your own laboratory, or send your samples to us and
take advantage of our PCR Array Services.
Product Support
Links
Technical Support
Presentations and Publications
Purchase Support
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