The Cellular Activation of Signaling ELISA or CASE Kits monitor the activation of signal transduction pathways by analyzing the phosphorylation of their upstream regulators. Each CASE™ Kit is a cell-based ELISA and includes a complete antibody-based detection system for the colorimetric quantification of the phosphorylated form and the total amount of a specific protein. A greater extent of phosphorylation indicates an increased stimulation of the corresponding downstream pathway. Use these kits to directly determine the extent of protein phosphorylation and pathway activation after treatment with a specific drug or inhibitor without the need to prepare cell lysates.

Quantitative: Cell-based ELISA determines level of total and activated form of protein at same time.

Simple: Easy, quantitative, and non-radioactive protocol with minimal hands-on time

No Extractions: Directly measure protein phosphorylation state on cells fixed in a 96-well culture plate

Learn more about CASE Kits, download our technology brochure [here].
For a complete overview of the CASE kit technology, click [here].

Features of the CASE™ Kits:

Western blot analysis and radioactive in vitro kinase assays have found widespread use in the monitoring of kinase activation. However, both methods suffer from distinct disadvantages. These tedious multi-step methods require a large amount of cells and treatment reagents, and are not suitable for high-throughput studies. They also involve making cell lysates, a process during which protein phosphorylation status can be lost.

CASE Kits overcome these difficulties, requiring no protein extraction and very little hands-on-time. Now you can assay phosphorylation of important proteins in a convenient 96-well plate format that is easily scaled up for screening experiments. The CASE kits may also be seen as high-throughput method for analyzing the phosphorylation status of specific proteins inside cells.

SuperArray provides a full range of solutions for your needs to study the function of genes:

• Find a CASE for your favorite protein or pathway by browsing through our Product Listing.
  
• Find the right kinase inhibitor to control for specificity in your assay [here].
  
• Read [this] about the reagents & equipment required to use the CASE Kit in your lab.
  
• For CASE Kit Pricing and other Ordering Instructions, click [here].