| Description |
The Mouse IL23 Single Analyte ELISArray Kit
is designed to quantitatively measure the amount of this cytokine in cell
culture supernatant, serum or plasma using a conventional enzyme-linked
immunosorbent assay (ELISA). Each 96-well plate (8 wells/strip, 12 strips)
is coated with the protein-specific capture antibody. We screened all
commercially available antibodies to identify the best capture and
detection antibodies. The high sensitivity, good linearity and low
background of the Single Analyte ELISArray Kits provide reliable and
reproducible results for your cytokine and chemokine analyses.
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| Product Resources |
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| More information on IL23 from Wikipedia: |
| "IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. In conjunction with IL-6 and TGF-β1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-alpha, NOS-2, and chemokines resulting in inflammation. Knockout mice deficient in either p40 or p19, or in either subunit of the IL-23 receptor (IL-23R and IL12R-β1) develop less severe symptoms of multiple sclerosis and inflammatory bowel disease highlighting the importance of IL-23 in the inflammatory pathway." |
| Kit Contents / Storage Conditions/
Shelf Life |
| Please check the kit components immediately after you receive this package. SuperArray is only responsible for missing items reported within two (2) business days of receipt.
Enough reagents are provided to process the included plate of 12 ELISA
strips.
| Component / Description |
Quantity |
| BOX 1: Shipped on blue ice packs. Store
at -20 ºC. |
| Antigen Standard (1 mg/ml) |
1.5-ml tube |
| Detection Antibody |
1.5-ml tube |
| Avidin-HRP Conjugate |
1.5-ml tube |
| 10% BSA |
15 ml bottle |
| Donkey Serum |
15 ml bottle |
| BOX 2: Shipped at ambient temperature.
Store at 4 ºC. |
| Pre-coated Capture Antibody 8-well strips |
One plate of 12 strips in a pouch |
| Sample Dilution Buffer Stock |
60 ml bottle |
| Assay Buffer Stock |
60 ml bottle |
| Wash Buffer (10X Concentrate) |
125 ml bottle |
| Development Solution |
60 ml bottle |
| Stop Solution |
60 ml bottle |
Storage Conditions: All reagents are stable at the
recommended temperature for 6 months after receipt of the kit. Do
not use kit beyond the expiration date printed on the label. |
| Brief Protocol |
The Brief Protocol is meant for experience
users only. First-time users should refer to the User
Manual.
- Prepare replicate serial dilutions of the Antigen Standard and your
experimental samples.
- Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA
strips.
- Transfer 50 µl samples and/or standards to the appropriate wells of
the ELISA strips.
- Gently shake or tap plate for 10 s. Incubate for 2 h at room temp.
- Washing ELISA Wells:
Decant or aspirate well contents. Add 350 µl 1X Washing Buffer.
Gently shake or tap plate for 10 s. Decant or aspirate. Blot array
upside down on absorbent paper to remove any residual buffer. Repeat
wash twice more.
- Pipette 100 µl of Detection Antibody solution. Incubate 1 h at room
temp.
- Wash ELISA wells as described above.
- Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 min at
room temp.
- Wash ELISA wells for a total of 4 washes.
- Add 100 µl of Development Solution to each well. Incubate the plate
for 15 min at room temp in the dark.
- Add 100 µl of Stop Solution to each well. The color changes from
blue to yellow.
- Read absorbance at 450 nm within 30 min of stopping the reaction. If
wavelength correction is available, subtract readings at 570 nm from
the reading at 450 nm.
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