| Description |
The Human IL1B Single Analyte ELISArray Kit
is designed to quantitatively measure the amount of this cytokine in cell
culture supernatant, serum or plasma using a conventional enzyme-linked
immunosorbent assay (ELISA). Each 96-well plate (8 wells/strip, 12 strips)
is coated with the protein-specific capture antibody. We screened all
commercially available antibodies to identify the best capture and
detection antibodies. The high sensitivity, good linearity and low
background of the Single Analyte ELISArray Kits provide reliable and
reproducible results for your cytokine and chemokine analyses.
|
|
|
| Product Resources |
|
|
| More information on IL1B from NCBI: |
| "The protein encoded by this gene is a member of the
interleukin 1 cytokine family. This cytokine is produced by activated
macrophages as a proprotein, which is proteolytically processed to its
active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator
of the inflammatory response, and is involved in a variety of cellular
activities, including cell proliferation, differentiation, and apoptosis.
The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the
central nervous system (CNS) is found to contribute to inflammatory pain
hypersensitivity. This gene and eight other interleukin 1 family genes form
a cytokine gene cluster on chromosome 2." |
| Kit Contents / Storage Conditions/
Shelf Life |
| Please check the kit components immediately after you receive this package. SuperArray is only responsible for missing items reported within two (2) business days of receipt.
Enough reagents are provided to process the included plate of 12 ELISA
strips.
| Component / Description |
Quantity |
| BOX 1: Shipped on blue ice packs. Store
at -20 ºC. |
| Antigen Standard (1 mg/ml) |
1.5-ml tube |
| Detection Antibody |
1.5-ml tube |
| Avidin-HRP Conjugate |
1.5-ml tube |
| 10% BSA |
15 ml bottle |
| Donkey Serum |
15 ml bottle |
| BOX 2: Shipped at ambient temperature.
Store at 4 ºC. |
| Pre-coated Capture Antibody 8-well strips |
One plate of 12 strips in a pouch |
| Sample Dilution Buffer Stock |
60 ml bottle |
| Assay Buffer Stock |
60 ml bottle |
| Wash Buffer (10X Concentrate) |
125 ml bottle |
| Development Solution |
60 ml bottle |
| Stop Solution |
60 ml bottle |
Storage Conditions: All reagents are stable at the
recommended temperature for 6 months after receipt of the kit. Do
not use kit beyond the expiration date printed on the label. |
| Brief Protocol |
The Brief Protocol is meant for experience
users only. First-time users should refer to the User
Manual.
- Prepare replicate serial dilutions of the Antigen Standard and your
experimental samples.
- Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA
strips.
- Transfer 50 µl samples and/or standards to the appropriate wells of
the ELISA strips.
- Gently shake or tap plate for 10 s. Incubate for 2 h at room temp.
- Washing ELISA Wells:
Decant or aspirate well contents. Add 350 µl 1X Washing Buffer.
Gently shake or tap plate for 10 s. Decant or aspirate. Blot array
upside down on absorbent paper to remove any residual buffer. Repeat
wash twice more.
- Pipette 100 µl of Detection Antibody solution. Incubate 1 h at room
temp.
- Wash ELISA wells as described above.
- Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 min at
room temp.
- Wash ELISA wells for a total of 4 washes.
- Add 100 µl of Development Solution to each well. Incubate the plate
for 15 min at room temp in the dark.
- Add 100 µl of Stop Solution to each well. The color changes from
blue to yellow.
- Read absorbance at 450 nm within 30 min of stopping the reaction. If
wavelength correction is available, subtract readings at 570 nm from
the reading at 450 nm.
|
