| Description |
The Human Th1 / Th2 / TH17 Cytokines Multi-Analyte ELISArray Kit analyzes a panel of 12 cytokines involved in T helper cell biology using a conventional ELISA protocol all at once under uniform conditions. The cytokines represented by this array are IL2, IL4, IL5, IL6, IL10, IL12, IL13, IL17A, IFNγ, TNFα, G-CSF, and TGFβ1. The Th1-related cytokines include IL2, IL10, IL12, IFNγ, and TNFα, while the Th2-related cytokines include IL2, IL4, IL5, IL10, and IL13. IL6, IL17A, TNFα, G-CSF, and TGFβ1 are all related to TH17 cells. The complete kit features the best possible combination of capture and detection antibodies for each protein in the panel and all of the needed colorimetric detection reagents. Using the same ELISA protocol and development or incubation time, you can easily profile the levels of a focused panel of 12 cytokines related to T helper cells with this array.
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| Product Resources |
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| Protein Panel: |
| IL2, IL4, IL5, IL6, IL10, IL12, IL13, IL17A, IFNγ, TNFα, G-CSF, TGFβ1
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| What is a Multi-Analyte ELISArray Kit? |
| The ELISArray Cytokine and Chemokine Kits are the simplest simultaneous multi-analyte enzyme-linked immunosorbent assays (ELISA) in the market. The ELISArray Kits are designed to survey a specific panel of cytokines or chemokines involved in autoimmunity, inflammation, or T-cell biology in cell culture supernatant, serum or plasma. The ELISArray Kits can be used for autoimmune and immune disorder, cancer, immunology, infectious disease, or any other area of biological and medical research normally using individual ELISA kits.
Need more information about ELISArray Kits?
Please visit ELISArray Kit home page or send
an Email to Technical Support. |
| Kit Contents / Storage Conditions/
Shelf Life |
| Please check the kit components immediately after you receive this package. SuperArray is only responsible for missing items reported within two (2) business days of receipt.
Enough reagents are provided to process the included plate of 12 ELISA
strips.
| Component / Description |
Quantity |
| BOX 1: Shipped on blue ice packs. Store
at -20 ºC. |
| BOX 1A: Antigen
Standards |
One box of 12 1.5-ml tubes |
| BOX 1B: Detection
Antibodies |
One box of 12 1.5-ml tubes |
| Avidin-HRP Conjugate |
One 1.5-ml tubes |
| 10% BSA |
15 ml bottle |
| Donkey Serum |
15 ml bottle |
| BOX 2: Shipped at ambient temperature.
Store at 4 ºC. |
| 96-well pre-coated Capture Antibody microplate |
One plate of 12 strips in a pouch |
| Detection Antibody Dilution Tube Strip |
One strip of 12 tubes |
| Sample Dilution Buffer Stock |
60 ml bottle |
| Assay Buffer Stock |
60 ml bottle |
| Wash Buffer (10X Concentrate) |
125 ml bottle |
| Development Solution |
60 ml bottle |
| Stop Solution |
60 ml bottle |
Storage Conditions: All reagents
are stable at the recommended temperature for 6 months after
receipt of the kit. Do not use kit beyond the expiration date
printed on the label. |
| Brief Protocol |
The Brief Protocol is meant for experience
users only. First-time users should refer to the User
Manual.
- Prepare replicate dilutions of your experimental samples.
- Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA
strips.
- Transfer 50 µl samples and/or standards to the appropriate wells of
the ELISA strips.
- Gently shake or tap plate for 10 s. Incubate for 2 h at room temp.
- Washing ELISA Wells:
Decant or aspirate well contents. Add 350 µl 1X Washing Buffer.
Gently shake or tap plate for 10 s. Decant or aspirate. Blot array
upside down on absorbent paper to remove any residual buffer. Repeat
wash twice more.
- Pipette 100 µl of Detection Antibody solution. Incubate 1 h at room
temp.
- Wash ELISA wells as described above.
- Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 min at
room temp.
- Wash ELISA wells for a total of 4 washes.
- Add 100 µl of Development Solution to each well. Incubate the plate
for 15 min at room temp in the dark.
- Add 100 µl of Stop Solution to each well. The color changes from
blue to yellow.
- Read absorbance at 450 nm within 30 min of stopping the reaction. If
wavelength correction is available, subtract readings at 570 nm from
the reading at 450 nm.
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